http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab The Biochem/Nachman_lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2013 by contributing authors Ido Gan [idoga@tauex.tau.ac.il] The contributing authors of TWiki TWiki http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab http://ibis.tau.ac.il/twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/IPSReversionProject EvgenyY 03 Apr 2013 Members Evgeny Yurkovsky Camille Sindhu Intoduction Differentiation of stem cells, as well as reprogramming of ... (last changed by EvgenyY) 2013-05-08T09:53:47Z EvgenyY http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/JuniorPIforum TAU young PI forum Introduction Upcoming meetings:(all at UK 222, 12:00) April 22 Prof Danny Chamovitz on "Everything you wanted to know about getting tenure ... (last changed by IftachN) 2013-03-04T08:46:44Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/Immunostaining InbarC 27 Jun 2011 Immunostaining protocol: 1. Wash 3x in PBSx1 2. Fix for 12 15 minutes in 4% Paraformaldehyde in PBSx1 3. Wash 3x in PBSx1 4. Permeabilization ... (last changed by InbarC) 2013-02-03T07:35:39Z InbarC http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/LowFluorescenceMedium Low Fluorescence Yeast Media Modified from BIO101 catalog: 20x salt stock: (NH4)2SO4 100g KH2PO4 20g MgSO4·7H20 10g NaCl 2g CaCl2·2H20 2g Bring to 1l with Milli Q ... (last changed by YifatGol) 2013-01-23T09:07:09Z YifatGol http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/GoodVibrations Good vibrations, good collaborations IftachN 13 Jan 2013 Negotiating good collaborations in the lab, by Noam Shomron. (last changed by IftachN) 2013-01-13T14:29:13Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/SplittingEScells FeederFreeTroubleshooting Differentiation medium: Same as ES media OR N2B27 butwithout Lif or other inhibitors (Chir, PD). Splitting/Seeding ES Cells 1. Warm up cell ... (last changed by IftachN) 2012-12-24T09:45:48Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/MediaRecipes mESC media ES Medium 250mL DMEM 217.5 ml 15% FBS (MEF) 37.5 mL L Glutamine (100x) 2.5 ml P/S (100x) 2.5 mL NEAA(100x) 2.5 mL B mercapto etanol 2ul (easier ... (last changed by IftachN) 2012-12-24T09:43:34Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/CellCulture Preparing feeders Working with ES/ips cells DailyMediumReplacement EB growth GrowingCellsInPDMS MediaRecipes IncubatorMaintenance ViralProduction DifferentiationOfPlatedEBs ... (last changed by InbarC) 2012-12-24T08:49:47Z InbarC http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/FedExShipments Fedex shipment to us fedex account number : 307646412 (old number: 266474148;abbreviated number: 24633; this does not work anymore for shipments from abroad ... (last changed by IftachN) 2012-12-11T21:47:59Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/LeavingTheLab Guidelines for checking out of the lab (adopted from Maya Schuldiner) General notes Start working on checking out about a week before your last day, or 2 3 weeks ... (last changed by IftachN) 2012-11-28T15:14:43Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/ComputerBackups Computer backups The lab share (also known as "S:" or /groups/iftach) is backed up to our QNAP server every Sunday. The wiki pages are being backed up by Life Sciences ... (last changed by IftachN) 2012-11-28T13:09:29Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/General_Procedures YifatGol 05 May 2010 FedEx Shipments Computer Backups Leaving the Lab (last changed by IftachN) 2012-11-28T13:08:40Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/EBGrowth IftachN 06 Jun 2010 EB formation cardiogenesis medium FeederFreeTroubleshooting (last changed by MaayanPour) 2012-11-26T08:18:28Z MaayanPour http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/RunningOnLecs Ssh to lecs: ssh lecs Running yeast image analysis pipeline If needed, edit these two lines in RUN PROCESS EXPERIMENT.m : actions 1 2 3 3 4 6 ... (last changed by IftachN) 2012-11-13T11:40:34Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/BasicLinux Basic linux commands here. Biocluster tutorial here. biodata (a.k.a. S:\) IftachN 13 Nov 2012 (last changed by IftachN) 2012-11-13T10:26:52Z IftachN http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman_lab/Computation BasicLinux RunningOnLecs CellProfiler Imaris IftachN 13 Nov 2012 (last changed by IftachN) 2012-11-13T10:04:20Z IftachN