50 Recent Changes in Biochem/Nachman_lab Web retrieved at 10:08 (GMT)

WebStatistics in Biochem.Nachman_lab
Statistics for nop Biochem/Nachman lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for ...
TrojanHorses in Biochem.Nachman_lab
Background Differentiation decisions are affected by local signals, such as Wnt, BMP, FGF. To evaluate the local effects of signals at specific decision points, we ...
CRISPR in Biochem.Nachman_lab
InbarC 23 Oct 2013 Oligo design: 1. Working with plasmid 335 (Addgene) that contains nickase chimeric guide RNA or 330 (single cut). We intend to work according ...
changed on 24 Sep 2015 - 08:44 by InbarC
XfectReagentForES in Biochem.Nachman_lab
Xfect™ mESC Transfection Reagent Use this kit according to manufacturer's instractions. Selection for positive cells can be done between 24 to 72 hours after transfection ...
changed on 24 Sep 2015 - 07:07 by InbarC
Transfection in Biochem.Nachman_lab
electroporation Xfect reagent for ES
changed on 24 Sep 2015 - 06:56 by InbarC
ViralProduction in Biochem.Nachman_lab
Protocol for LentiviralTransgene Delivery Use TransIT® LT1 according to manufacturer's instructions (Mirus https://www.mirusbio.com/assets/protocols/ml001 transit ...
changed on 24 Sep 2015 - 06:54 by InbarC
IPSReversionProjectRNS-seqAnalysis in Biochem.Nachman_lab
! Set ALLOWTOPICCHANGE .NachmanGroup, .CamilleSindhu EvgenyY 03 Apr 2013 Members Evgeny Yurkovsky Camille Sindhu Data analysis Gene expression ...
General_Procedures in Biochem.Nachman_lab
YifatGol 05 May 2010 FedEx Shipments Computer Backups Leaving the Lab Kravitz TAU Address
changed on 29 May 2014 - 07:42 by InbarC
TAUAddress in Biochem.Nachman_lab
InbarC 29 May 2014 Tel Aviv University Levanon street Ramat Aviv Department of Biochemistry and Molecular Biology Sherman Building, Room 506 Tel Aviv, 69978 ISRAEL ...
changed on 29 May 2014 - 07:41 by InbarC
Kravitz in Biochem.Nachman_lab
InbarC 11 May 2014 Account number 2223627 Tel: 08 914777 Fax: 08 9232020
changed on 11 May 2014 - 11:08 by InbarC
Gibson in Biochem.Nachman_lab
InbarC 18 Mar 2014 Gibson assay 1. Design primers for up to 5 fragments of plasmid and insert. (Recommend inside the vector design primers in the selection marker ...
changed on 18 Mar 2014 - 10:13 by InbarC
LiOAc in Biochem.Nachman_lab
Protocol for: EXTRACTION OF GENOMIC DNA FROM YEASTS REAGENTS: 1.0.2 M Lithium acetate 1% SDS solution. 2.Ethanol 96 100 % and 70 %. PROCEDURE: 1.Pick one yeast colony ...
changed on 26 Nov 2013 - 09:53 by InbarC
FedExShipments in Biochem.Nachman_lab
Fedex shipment to us fedex account number : 307646412 abbreviated number: 24633 Our address: Department of Biochemistry The George S. Wise Faculty of Life Sciences ...
changed on 16 Oct 2013 - 14:28 by InbarC
OptoGenetics in Biochem.Nachman_lab
Optical activation of genes in order to manipulate cell differentiation Background Understanding and controlling stem cells differentiation holds a major scientific ...
MycoplasmaTest in Biochem.Nachman_lab
InbarC 10 Sep 2013 EZ PCR Mycoplasma test kit cat no. 20 700 20 (biological industries http://www.bioind.com/Media/Uploads/ez pcr 20 700 20(1).pdf
changed on 10 Sep 2013 - 06:46 by InbarC
CellCulture in Biochem.Nachman_lab
Preparing feeders Working with ES/ips cells DailyMediumReplacement EB growth GrowingCellsInPDMS MediaRecipes IncubatorMaintenance ViralProduction DifferentiationOfPlatedEBs ...
changed on 10 Sep 2013 - 06:42 by InbarC
ReprogrammingExperiment in Biochem.Nachman_lab
InbarC 29 Aug 2013 Protocol for reprogramming experiment of secondary MEFs (Zack) 1. Split one day before infection (1.5 10^5 at 12 well). 2. Infection of ~200,000 ...
changed on 29 Aug 2013 - 09:50 by InbarC
CheckCheck in Biochem.Nachman_lab
TomShachar 20 Aug 2013 PCR using Platinum pfx polymerase Protocol link: platinumpfx.pdf
GenralPCRReaction in Biochem.Nachman_lab
TomShachar 18 Aug 2013 Genral PCR reaction: Ingredients per test tube (10% surplus): 1.1 µL MgSO4 1.1 µL dNTP 5.5 µL AMP buffer 38.3 µL H2O Reaction steps: Step 1 ...
People in Biochem.Nachman_lab
Iftach Nachman Inbar Pilzer Yifat Goldshmidt Jonathan Boxman Amit Reif Daniel Dar Evgeny Yurkovsky Tom Shachar
LB-Agar in Biochem.Nachman_lab
TomShachar 18 Aug 2013 Lysogeny broth Agar preparation: Ingredients: 1. 800mL distilled H2O. 2. 10gr Bacto tryptone. 3. 5gr yeast extract. 4. 10 gr NaCl. 5. NaOH ...
AgarosePreparation in Biochem.Nachman_lab
TomShachar 18 Aug 2013 Agarose gel preparation: Ingredients per 8 holes template for 16 holes just double the amount 0.5 g Agarose. 50 mL TEA. 1 Drop ( 0.05 mL) ...
IPSReversionProject in Biochem.Nachman_lab
EvgenyY 03 Apr 2013 Members Evgeny Yurkovsky Camille Sindhu RNA seq data analysis http://ibis.tau.ac.il/twiki/bin/view/Biochem/Nachman ...
ISSCR2013-June2013,Boston in Biochem.Nachman_lab
ISSCR 11, June 12 15 2013, Boston This huge conference ( 4000 participants) had several plenary and many parallel sessions. Below are sample notes from some talks ...
ConferenceReports in Biochem.Nachman_lab
Live imaging forum March 2010, Givat Ram ISSCR 2013 June 2013, Boston IftachN 16 Mar 2010
Ampicillin in Biochem.Nachman_lab
stock: 10mg/ml: 500mg in 50ml sterile water in 50ml tube filter in blue (0.2) filter, and split to aliquots. To 400ml LB add 4ml Amp from the stock. (1:100) MaayanPour ...
DifficultConversations in Biochem.Nachman_lab
IftachN 21 May 2013 7 Tips for Difficult Conversations Difficult Conversations Nine Common Mistakes
JuniorPIforum in Biochem.Nachman_lab
TAU young PI forum Introduction Upcoming meetings:(all at UK 222, 12:00) None planned currently. Volunteer! Past Meetings Having Difficult Conversations ...
Immunostaining in Biochem.Nachman_lab
InbarC 27 Jun 2011 Immunostaining protocol: 1. Wash 3x in PBSx1 2. Fix for 12 15 minutes in 4% Paraformaldehyde in PBSx1 3. Wash 3x in PBSx1 4. Permeabilization ...
changed on 03 Feb 2013 - 07:35 by InbarC
LowFluorescenceMedium in Biochem.Nachman_lab
Low Fluorescence Yeast Media Modified from BIO101 catalog: 20x salt stock: (NH4)2SO4 100g KH2PO4 20g MgSO4·7H20 10g NaCl 2g CaCl2·2H20 2g Bring to 1l with Milli Q ...
GoodVibrations in Biochem.Nachman_lab
Good vibrations, good collaborations IftachN 13 Jan 2013 Negotiating good collaborations in the lab, by Noam Shomron.
SplittingEScells in Biochem.Nachman_lab
FeederFreeTroubleshooting Differentiation medium: Same as ES media OR N2B27 butwithout Lif or other inhibitors (Chir, PD). Splitting/Seeding ES Cells 1. Warm up cell ...
MediaRecipes in Biochem.Nachman_lab
mESC media ES Medium 250mL DMEM 217.5 ml 15% FBS (MEF) 37.5 mL L Glutamine (100x) 2.5 ml P/S (100x) 2.5 mL NEAA(100x) 2.5 mL B mercapto etanol 2ul (easier ...
LeavingTheLab in Biochem.Nachman_lab
Guidelines for checking out of the lab (adopted from Maya Schuldiner) General notes Start working on checking out about a week before your last day, or 2 3 weeks ...
ComputerBackups in Biochem.Nachman_lab
Computer backups The lab share (also known as "S:" or /groups/iftach) is backed up to our QNAP server every Sunday. The wiki pages are being backed up by Life Sciences ...
EBGrowth in Biochem.Nachman_lab
IftachN 06 Jun 2010 EB formation cardiogenesis medium FeederFreeTroubleshooting
RunningOnLecs in Biochem.Nachman_lab
Ssh to lecs: ssh lecs Running yeast image analysis pipeline If needed, edit these two lines in RUN PROCESS EXPERIMENT.m : actions 1 2 3 3 4 6 ...
BasicLinux in Biochem.Nachman_lab
Basic linux commands here. Biocluster tutorial here. biodata (a.k.a. S:\) IftachN 13 Nov 2012
Computation in Biochem.Nachman_lab
BasicLinux RunningOnLecs CellProfiler Imaris IftachN 13 Nov 2012
HowToSayNo in Biochem.Nachman_lab
How to improve time management / save time Establish "closed door" periods Work away from lab on deadlines 2 person "work date" (Roy BB) Two ...
Transformation in Biochem.Nachman_lab
Preperation competent cells: a. Heat shock The Inoue Method for Preparation of Competent E. coli Reference: Molecular Cloning 1.112 Pick a single bacterial colony ...
changed on 14 Oct 2012 - 09:42 by InbarC
DailyMediumReplacement in Biochem.Nachman_lab
20 minutes before : Turn on hood : press "ON" button for a few seconds; press "UV" button for a few seconds; beeping should stop in a few seconds; After ...
DifferentiationToCM in Biochem.Nachman_lab
Differentiation to get Brach For differentiation, ESCs were dissociated and cultured in suspension in serum free differentiation (SF D) media without additional growth ...
changed on 28 May 2012 - 15:45 by InbarC
GettingStudentsToWrapUp in Biochem.Nachman_lab
Meeting led by Maya Schuldiner (WIS). Different obstacles from wrapping up a project: Possible solutions: IftachN 16 May 2012
G418DropoutPlates in Biochem.Nachman_lab
The standard nitrogen sournce in YNB is Ammonium Sulfate. It inhibits the action of the G418 or cloNAT antibiotics, and is therefore replaced in this recipe by monosodium ...
DealingWithRejections in Biochem.Nachman_lab
Positive psychology dealing with rejections/failures by Neta E. YoungPI 17 Jan 2012
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r1 - 15 Nov 2006 - 19:43:52 - TWikiContributor
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