50 Recent Changes in Biochem/Nachman_lab Web retrieved at 12:35 (GMT)
DifficultConversations
in Biochem.Nachman_lab
IftachN 21 May 2013 7 Tips for Difficult Conversations Difficult Conversations Nine Common Mistakes
changed on 21 May 2013 - 12:17 by IftachN
JuniorPIforum
in Biochem.Nachman_lab
TAU young PI forum Introduction Upcoming meetings:(all at UK 222, 12:00) None planned currently. Volunteer! Past Meetings Having Difficult Conversations ...
changed on 21 May 2013 - 12:11 by IftachN
IPSReversionProject
in Biochem.Nachman_lab
EvgenyY 03 Apr 2013 Members Evgeny Yurkovsky Camille Sindhu Intoduction Differentiation of stem cells, as well as reprogramming of ...
changed on 08 May 2013 - 09:53 by EvgenyY
Immunostaining
in Biochem.Nachman_lab
InbarC 27 Jun 2011 Immunostaining protocol: 1. Wash 3x in PBSx1 2. Fix for 12 15 minutes in 4% Paraformaldehyde in PBSx1 3. Wash 3x in PBSx1 4. Permeabilization ...
changed on 03 Feb 2013 - 07:35 by InbarC
LowFluorescenceMedium
in Biochem.Nachman_lab
Low Fluorescence Yeast Media Modified from BIO101 catalog: 20x salt stock: (NH4)2SO4 100g KH2PO4 20g MgSO4ˇ7H20 10g NaCl 2g CaCl2ˇ2H20 2g Bring to 1l with Milli Q ...
changed on 23 Jan 2013 - 09:07 by YifatGol
GoodVibrations
in Biochem.Nachman_lab
Good vibrations, good collaborations IftachN 13 Jan 2013 Negotiating good collaborations in the lab, by Noam Shomron.
changed on 13 Jan 2013 - 14:29 by IftachN
SplittingEScells
in Biochem.Nachman_lab
FeederFreeTroubleshooting Differentiation medium: Same as ES media OR N2B27 butwithout Lif or other inhibitors (Chir, PD). Splitting/Seeding ES Cells 1. Warm up cell ...
changed on 24 Dec 2012 - 09:45 by IftachN
MediaRecipes
in Biochem.Nachman_lab
mESC media ES Medium 250mL DMEM 217.5 ml 15% FBS (MEF) 37.5 mL L Glutamine (100x) 2.5 ml P/S (100x) 2.5 mL NEAA(100x) 2.5 mL B mercapto etanol 2ul (easier ...
changed on 24 Dec 2012 - 09:43 by IftachN
CellCulture
in Biochem.Nachman_lab
Preparing feeders Working with ES/ips cells DailyMediumReplacement EB growth GrowingCellsInPDMS MediaRecipes IncubatorMaintenance ViralProduction DifferentiationOfPlatedEBs ...
changed on 24 Dec 2012 - 08:49 by InbarC
FedExShipments
in Biochem.Nachman_lab
Fedex shipment to us fedex account number : 307646412 (old number: 266474148;abbreviated number: 24633; this does not work anymore for shipments from abroad ...
changed on 11 Dec 2012 - 21:47 by IftachN
LeavingTheLab
in Biochem.Nachman_lab
Guidelines for checking out of the lab (adopted from Maya Schuldiner) General notes Start working on checking out about a week before your last day, or 2 3 weeks ...
changed on 28 Nov 2012 - 15:14 by IftachN
ComputerBackups
in Biochem.Nachman_lab
Computer backups The lab share (also known as "S:" or /groups/iftach) is backed up to our QNAP server every Sunday. The wiki pages are being backed up by Life Sciences ...
changed on 28 Nov 2012 - 13:09 by IftachN
General_Procedures
in Biochem.Nachman_lab
YifatGol 05 May 2010 FedEx Shipments Computer Backups Leaving the Lab
changed on 28 Nov 2012 - 13:08 by IftachN
EBGrowth
in Biochem.Nachman_lab
IftachN 06 Jun 2010 EB formation cardiogenesis medium FeederFreeTroubleshooting
changed on 26 Nov 2012 - 08:18 by MaayanPour
RunningOnLecs
in Biochem.Nachman_lab
Ssh to lecs: ssh lecs Running yeast image analysis pipeline If needed, edit these two lines in RUN PROCESS EXPERIMENT.m : actions 1 2 3 3 4 6 ...
changed on 13 Nov 2012 - 11:40 by IftachN
BasicLinux
in Biochem.Nachman_lab
Basic linux commands here. Biocluster tutorial here. biodata (a.k.a. S:\) IftachN 13 Nov 2012
changed on 13 Nov 2012 - 10:26 by IftachN
Computation
in Biochem.Nachman_lab
BasicLinux RunningOnLecs CellProfiler Imaris IftachN 13 Nov 2012
changed on 13 Nov 2012 - 10:04 by IftachN
HowToSayNo
in Biochem.Nachman_lab
How to improve time management / save time Establish "closed door" periods Work away from lab on deadlines 2 person "work date" (Roy BB) Two ...
changed on 12 Nov 2012 - 12:05 by IftachN
Transformation
in Biochem.Nachman_lab
Preperation competent cells: a. Heat shock The Inoue Method for Preparation of Competent E. coli Reference: Molecular Cloning 1.112 Pick a single bacterial colony ...
changed on 14 Oct 2012 - 09:42 by InbarC
DailyMediumReplacement
in Biochem.Nachman_lab
20 minutes before : Turn on hood : press "ON" button for a few seconds; press "UV" button for a few seconds; beeping should stop in a few seconds; After ...
changed on 18 Jul 2012 - 07:57 by IftachN
DifferentiationToCM
in Biochem.Nachman_lab
Differentiation to get Brach For differentiation, ESCs were dissociated and cultured in suspension in serum free differentiation (SF D) media without additional growth ...
changed on 28 May 2012 - 15:45 by InbarC
GettingStudentsToWrapUp
in Biochem.Nachman_lab
Meeting led by Maya Schuldiner (WIS). Different obstacles from wrapping up a project: Possible solutions: IftachN 16 May 2012
changed on 16 May 2012 - 06:15 by IftachN
WebStatistics
in Biochem.Nachman_lab
Statistics for nop Biochem/Nachman lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for ...
changed on 14 Apr 2012 - 22:42 by TWikiGuest
G418DropoutPlates
in Biochem.Nachman_lab
The standard nitrogen sournce in YNB is Ammonium Sulfate. It inhibits the action of the G418 or cloNAT antibiotics, and is therefore replaced in this recipe by monosodium ...
changed on 19 Mar 2012 - 14:13 by IftachN
DealingWithRejections
in Biochem.Nachman_lab
Positive psychology dealing with rejections/failures by Neta E. YoungPI 17 Jan 2012
changed on 18 Jan 2012 - 07:21 by YoungPI
ImprovingYourDepartment
in Biochem.Nachman_lab
Update: drawing money from the Maanak through a pension account (Shai Avidan) It is possible to draw money from the Maanak Klita into a pension savings account opened ...
changed on 11 Jan 2012 - 08:19 by IftachN
November2011Update
in Biochem.Nachman_lab
Statues: Transfection/selection front: issues resolved. Nucleofector works. Differentiation markers: Promoter marker validity not yet confirmed. New six month goals ...
changed on 03 Jan 2012 - 12:10 by InbarC
ViralProduction
in Biochem.Nachman_lab
Protocol for LentiviralTransgene Delivery (courtesy of the Meissner Lab) Day 1: Preparation of 293T cells 1. Split 293 T cells onto 15 centimeter dishes in 20 ml ...
changed on 07 Dec 2011 - 10:39 by InbarC
Purchasing,Pension,Etc
in Biochem.Nachman_lab
Assorted short topics Roy Beck GIF it is possible to list recommended reviewers, even though it is not on the form. Uri Gophna update status on the pension ...
changed on 05 Dec 2011 - 21:09 by IftachN
InductionProtocol
in Biochem.Nachman_lab
1. Trypsinize and aggregate cells into EBs for 48 hours (2 10^6 for six well or 500/20ul in hanging drop). 2. plate EBs/do not plate and change medium to add: a. BMP4 ...
changed on 29 Nov 2011 - 14:22 by InbarC
PlasmidStatues
in Biochem.Nachman_lab
Plasmid statues table InbarC 29 Nov 2011
changed on 29 Nov 2011 - 14:09 by InbarC
TableAndConfigurationOfPrimers
in Biochem.Nachman_lab
Attachments new style primers.xls: new style primers.xls Gibson lift.ape: Gibson lift.ape
changed on 29 Nov 2011 - 14:07 by InbarC
AroundTheHornPlasmidModification
in Biochem.Nachman_lab
1. PCR according to your needs (one primer with the modificaion, the other without. approx. 1 minute elongation for every 1kb). will take several hours. 2. Incubate ...
changed on 04 Oct 2011 - 11:35 by AmitReif
AroundTheHorn
in Biochem.Nachman_lab
AmitReif 19 Sep 2011
changed on 19 Sep 2011 - 12:30 by AmitReif
RC-8Spinner
in Biochem.Nachman_lab
RC8 spinner protocol Preparation and cleanliness: 1. prepare wafer by prebaking for 10min' at 200C' 2. HMOS protocol 007. 3. Cleanliness notes use paper to catch ...
changed on 07 Sep 2011 - 10:40 by InbarC
CleanRoomProtocols
in Biochem.Nachman_lab
1. Turn on the Hood 2. Turn on the Mask aligner 3. Spray the wafer with acetone IPA, dry in Microwave for 5min. 4. Prepare Spinner. 5. Pour SU8 on wafer CAREFULY ...
changed on 07 Sep 2011 - 10:33 by InbarC
PlasmidExtraction
in Biochem.Nachman_lab
Yeast Plasmid extraction protocol 1. Grow 5ml yeast cell culture Overnight. 2. Centrifuge for 5 min at 3000 rpm and discard supernatant. 3. Re suspend cell pellet ...
changed on 04 Sep 2011 - 09:11 by DanielDa
Nuclearstaininginlivecells(Hoechst33342)
in Biochem.Nachman_lab
1 Take 1.5 ml of a steady state culture in eppendorf and spin 5 sec at min at max speed 1 Resuspend pellet in 1ml solution A 1 Add 2ug Hoechst 33342 1 Stain ...
changed on 24 Jul 2011 - 05:55 by DanielDa
PlasmidTransformation
in Biochem.Nachman_lab
Plasmid Transformation (Sessa lab) 1. Grow yeast at 30ēC over night. 2. Wash with 5 ml sterile DDW. 3. Resuspend entire culture in LiAC TE solution. 4. Transfer 100 ...
changed on 19 Jul 2011 - 15:04 by DanielDa
IncubatorMaintenance
in Biochem.Nachman_lab
Tips from Arik Hasson (KadimaStem) Incubator cleaning Every 1 2 months in industry use Pharmacidal (Beit Haemek) based on quarternary benzylammonium ...
changed on 20 Jun 2011 - 11:21 by IftachN
MiRNAexpression
in Biochem.Nachman_lab
Summary Hypothesis: miRNAs work in groups, regulating groups of genes in a coordinated manner. A transcriptional response is achieved not by a small set of gene specific ...
changed on 19 Jun 2011 - 10:06 by IftachN
JonathanBoxman
in Biochem.Nachman_lab
DailyLog to do Target Statues statues Grow MEF cells, 1st take failure. Cells grew but after Mitomycin inactivation developed fungus on plate.lessons ...
changed on 14 Jun 2011 - 10:16 by IftachN
GettingThingsDoneAtTAU
in Biochem.Nachman_lab
IftachN 14 Jun 2011 A meeting with Prof. Karen Avraham Getting things done at TAU: summary slide Some bullit points: Startup funds: the part of your startup ...
changed on 14 Jun 2011 - 09:24 by IftachN
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