Same as my ligations- they ain't working!
Line: 7 to 6
Gal suggsestions- cut my plasmids with less material in template.
ligation problem solved. First construct (MBP insert with mfe-Xbai-MluI-Bam restriction sites) prepared.
plan to 14.5:
a. prepare construct of YFP1, YFP2, YFP.
Thursday 2.6: PCR, restriction, DP.
Friday 3.6: ligation, transformation.
Saturday 4.6: place for mini.
Sunday 5.6: Sequencing.
Tuesday 7.6: Meet with Gal, discuss next step, setup meeting with Ben-Tal.
-- InbarC - 07 Mar 2011
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